A Study on the Antioxidant and Active Compounds of <em>Gardenia jasminoides</em> Ellis (GJE) Leaves Extract

Purpose: The aim of this study was to isolate and identify the active compound antioxidant fractions from Gardenia jasminoides Ellis leaves. Research Method: activity determined using DPPH 0.1 mM (1:1), analyzed spectrophotometrically. In addition, separation performed column chromatography. most identified by UV-Vis, FT-IR, LC-MS/MS. quantitative analysis done in three replicated. Findings: It discovered that semi-polar fraction (IC50 = 62,50 ppm) higher than both polar non-polar fractions. Combining 295 isolates via chromatography, 11 combined were obtained (fA-fK). fK had highest 90.38% IC50 15.74 ppm. also 2 maximum wavelengths (336 275 nm) could be categorised as flavonoid (flavonol with 3-OH substituted). FTIR showed functional groups found flavonoid. Finally, identification LC-MS/MS at retention times 5,91 5,06 minutes kaempferol 3-O-rutinoside rutin, respectively. Originality/ Value: Based on results, it concluded GJE leaves have potential developed a new inexpensive source flavonoids.